胎次对奶山羊胎盘提取物抗氧化活性影响

doi:10.11843/j.issn.0366-6964.2017.08.007

摘要/Abstract

摘要：

旨在探讨不同胎次的奶山羊胎盘提取物抗氧化活性的差异，为其合理化应用提供技术依据。以1，1-二苯基-2-三硝基苦肼自由基（DPPH·）清除率、羟自由基（·OH）清除率为抗氧化肽活性评价指标，结合胎盘提取物中可溶性蛋白（Water-soluble protein，WSP）含量变化，比较不同存放条件随时间延长各指标的衰变情况，评价不同胎次羊胎盘提取物抗氧化活性优劣。结果表明：1）第1、2、3胎各胎盘提取物冷冻干燥后，其多肽含量分别为（0.201±0.03）、（0.270±0.02）、（0.193±0.02）mg·mg^-1冻干粉；DPPH·清除率分别为32.75%、53.20%、37.97%；·OH清除率分别是35.45%、51.94%、39.35%；WSP含量分别为16.63%、23.87%、18.60%，表明第2胎胎盘提取物各指标最佳。2）各胎次提取物4℃保存时，随时间延长DPPH·清除率、·OH清除率、WSP含量3个指标保全率均明显优于常温存放（P<0.05）。3）4℃存放63 d，第2胎提取物DPPH·清除率保全了78.85%；·OH清除率保全了71.93%；WSP含量保全率为77.36%。综上表明，第2胎胎盘提取物冷冻干燥，4℃存放比常温保存抗氧化活性好。

Abstract:

The objective of this work was to explore the impact of parities on antioxidant activities of extractions from dairy goat placenta, which could provide technical foundation to the application of placenta. The scavenging rate of DPPH radical and hydroxyl radical were taken as the evaluate indices of antioxidant peptides activity, the water-soluble protein content in the extract was also taken into account. The changes of placenta extract indices for different parities were compared and the attenuation of indicators with the change of storage conditions was investigated. The results showed that:1) The polypeptides contents of 1^st, 2^nd, 3^rd parities in placenta were (0.201±0.03), (0.270±0.02) and (0.193±0.02) mg·mg^-1 dry matter, respectively. The DPPH radical scavenging rate of 1^st, 2^nd, 3^rd parities were 32.75%, 53.20% and 37.97%.The scavenging rate of hydroxyl radicals of these 3 parities were 35.45%, 51.94% and 39.35%. The water-soluble protein content were 16.63%, 23.87% and 18.60%, respectively. 2) Compared with the ambient temperature, the decay rate was slower under the condition of 4℃, there was significant difference among parities under different conditions (P<0.05). 3) The preservation rate of DPPH radical scavenging of 2^nd parities at 4℃ was 78.85%.The preservation rate of hydroxyl radicals scavenging activity of 2^nd parities was 71.93%. The preservation rate of the water-soluble protein content was 77.36%. In conclusion, it is demonstrated that the 2^nd parities was the most prominent on antioxidant activity, dacay rate and WSP content and 4℃ was the ideal storage temperature for the preservation of activities.

中图分类号:

S826.2

高闪闪, 葛武鹏, 裴剑飞, 王西宁, 李小鹏, 梁秀珍, 吴小勇. 胎次对奶山羊胎盘提取物抗氧化活性影响[J]. 畜牧兽医学报, 2017, 48(8): 1436-1445.

GAO Shan-shan, GE Wu-peng, PEI Jian-fei, WANG Xi-ning, LI Xiao-peng, LIANG Xiu-zhen, WU Xiao-yong. Effect of Different Parities on the Antioxidant Activity of Dairy Goat Placenta Extract[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1436-1445.

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https://www.xmsyxb.com/CN/Y2017/V48/I8/1436

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